A Dual-Type L2 11-88 Peptide from HPV Types 16/18 Formulated in Montanide ISA 720 Induced Strong and Balanced Th1/Th2 Immune Responses, Associated with High Titers of Broad Spectrum Cross-Reactive Antibodies in Vaccinated Mice

Figure 3

Cross-reactive antibody responses of three dual-type fusion peptides against protein L2 11-200 from various HPV types. Mice were immunized 3 times at two-week interval with dual-type peptides emulsified in CFA/IFA adjuvants. Two weeks after the last vaccination, blood samples were collected, and twofold serially diluted sera were prepared (starting dilution at 1 : 100) and antibody cross-reactive titers against HPV 16 L2 11-200 (a), HPV 18 (b), HPV 31 (c), and HPV 45 (d) were determined by end point dilution ELISA. Data are represented as means ± standard error of means (SEM) of triplicates from 5 mice per group. , , , and . ns: not significant.

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