Markers of stem cells and epithelial-mesenchymal transition present in cervical cancer cells. (a) Expression of CD117 (c-kit) on INBL, HeLa, CaSki, and C33A cells was determined using anti-CD117 antibodies conjugated with PE-Cy7. The upper graphics indicating granularity versus fluorescence in PE-Cy7 show the staining controls for each cell line, and the lower graphics indicating granularity versus CD117 PE-Cy7 show the positive cells stained for CD117. (b) Expression of CD24 and CD44 on the INBL, HeLa, CaSki, and C33A cells was determined using anti-CD24 antibodies conjugated with PE and anti-CD44 antibodies conjugated with FITC. The upper dot plots show the staining controls for each cell line, and the lower dot plots show the double-positive cells stained for CD24 and CD44. For C33A cells, only CD24-positive cells were found.