Absence of Nonclassical Monocytes in Hemolytic Patients: Free Hb and NO-Mediated Mechanism

Figure 5

High NO level in monocytes of PNH/SCD patients correlates with 7AAD positivity. Monocytes from PNH ()/SCD () patients (the same patients mentioned in Figure 1) and healthy individuals () were stained with DAF-FM-DA dye for measuring NO levels or 7-AAD dye for assessing cell death using flow cytometry. (a) Scatter dotplot showing expression levels of NO. (b) A representative histogram showing the mean fluorescence intensity differences in PNH/SCD patients and healthy individuals. (c) A dotplot showing the percentage of 7-AAD-positive cells from PNH/SCD patients and healthy individuals. (d) A correlation plot showing the positive correlation between NO levels and cell death of monocytes from PNH/SCD patients. Each dot represents percent-positive cells for an individual. The Mann–Whitney test was used for the comparison between the groups. .

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