Research Article

pDC Activation by TLR7/8 Ligand CL097 Compared to TLR7 Ligand IMQ or TLR9 Ligand CpG

Figure 1

Ligands of TLR7 can induce a dramatic morphological change in pDCs. We harvested pDCs after activation with TLR7 ligands (IMQ, CL097, and CL075) and TLR9 ligands (CpG A, CpG B, and CpG C) for 24, 48, and 72 hours. The concentration for each ligand was 1.5 μM. We then assessed pDCs for morphological changes by Giemsa staining. Representative images from one of three experiments were shown. For each stained section, at least three images from random fields were taken. Magnification was ×500 (oil immersion).