43 kDa Glycoprotein (gp43) from Paracoccidioides brasiliensis Induced IL-17A and PGE2 Production by Human Polymorphonuclear Neutrophils: Involvement of TLR2 and TLR4

Figure 4

Involvement of TLR2 and TLR4 in the production of IL-6 (a), IL-10 (b), IL-12 (c), IFN-γ (d), and TNF-α (e) by human polymorphonuclear neutrophils (PMN) stimulated or not with gp43 from P. brasiliensis. Cells were incubated in the absence or presence of anti-TLR2 and anti-TLR4 antibodies, individually or in combination, for 2 h. PMNs were then stimulated or not with gp43 (20 ng/ml) during 4 h, and cytokine concentrations (pg/ml) were measured in the supernatant culture. Results are expressed as median using data from 7 women tested, considering .

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