Expression of LAIR-1 (CD305) on Human Blood Monocytes as a Marker of Hepatic Cirrhosis Progression
Effect of activation on LAIR-1 expression in healthy monocytes in vitro. The expression of LAIR-1 was analyzed by Western blotting in total protein extracts from isolated human healthy monocytes () unstimulated (control) or stimulated with LPS or C. albicans 1 : 5 cell : yeast ratio. A representative Western blot is shown (a). The bands were quantified by densitometry and normalized to β-actin. (b). LAIR-1 expression was analyzed by flow cytometry after LAIR-1 and CD14 staining. Representative histograms of monocytes unstained and stained with LAIR-1 antibody in isolated monocytes are shown (c). The cell surface LAIR-1 protein expression was quantified by measuring the MFI (median fluorescence intensity) after cell staining, and the effect of the activation with LPS or C. albicans was analyzed (d). Data represented in histograms are the mean and SEM. Student’s -test: .
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