IFN-γ Reduces Epidermal Barrier Function by Affecting Fatty Acid Composition of Ceramide in a Mouse Atopic Dermatitis Model

Figure 2

Cytokine expression in IMQ-induced dermatitis: no change in IFN-γ. IMQ cream was applied daily to the auricles of BALB/c mice for 8 times. As control, nothing was applied. Twenty-four hours after the eighth application on day 7, total RNA was extracted from the whole auricles and the expression of cytokines was determined by quantitative RT-PCR. “IL-12/IL-23” means IL-12 and/or IL-23 because p40, a common subunit to IL-12 and IL-23, was amplified. Expression of each cytokine is presented as a value relative to GAPDH mRNA. Data are presented as in six mice per group and are from one experiment representative of two independent experiments. Two-sided Student’s t-test was used for pair-wise comparisons. and .