Low-Dose Irradiation Differentially Impacts Macrophage Phenotype in Dependence of Fibroblast-Like Synoviocytes and Radiation Dose

Figure 4

Irradiation with 0.5 Gy alters fibroblast-like synoviocyte supernatant-stimulated macrophage phenotype. hTNF-α tg bone marrow cells were isolated and differentiated with 5 ng/ml M-CSF into M0 macrophages according to the experimental set-up shown in (a). Prior to macrophage generation, fibroblast-like synoviocyte (FLS) cultures were generated and phenotyped. SN of FLS were collected and stored at -80°C until they were added to macrophage cultures 24 h prior to characterization of the macrophage phenotype and 2 h prior to irradiation with the displayed doses of X-rays. The M2 macrophage marker CD206 (b) was significantly increased after irradiation with 0.5 Gy. M1 macrophage markers CD80 (c) and CD86 (e) showed a slightly reduced surface expression again after irradiation with 0.5 Gy, while the expression of MHCII (D) was not altered. Depicted is data from three independent experiments, each performed in triplicate (data points from one experiment are marked with equal symbols). Data is presented as the . . Representative histograms of the analyses are displayed in Supplementary Figure 4B.

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