Research Article

Enhancement of Immune Responses by Guanosine-Based Particles in DNA Plasmid Formulations against Infectious Diseases

Table 1

Mouse groups for the immunization studies.

Mouse groupsDNA plasmid (μg)SHS particles (mM)

Naïve00
pVAX11000
SHS00.1515
pGag1000
pGag+SHS1000.1515
pA27L1000
pA27L+SHS1000.1515
pOD1A27Lopt1000
pOD1A27Lopt+SHS1000.1515

The dosages of injections for experimental groups (pDNAs alone or pDNAs+SHS) were as follows: 50 μL per leg of pDNA preparation (1 μg/μL of pDNA in 100 μL PBS) or 50 μL per leg of pDNA+SHS preparation (1 μg/μL of of SHS in 100 μL PBS). Control mouse groups included those to determine basal responses (naïve) and those receiving 50 μL per leg of backbone plasmid (1 μg/μL pVAX1 in 100 μL PBS) and 50 μL per leg of SHS particles alone (0.1515 mM SHS in 100 μL PBS) to demonstrate nonantigen-specific immune responses.