Journal of Immunology Research / 2019 / Article / Tab 1

Research Article

Human Platelet Lysate Media Supplement Supports Lentiviral Transduction and Expansion of Human T Lymphocytes While Maintaining Memory Phenotype

Table 1

Results of immunostaining/flow cytometry following expansion.

DonorCD3+CD4+ (percent)CD3+CD8+ (percent)CD3+CD56-(percent)CD3+CD56+ (percent)CD3-CD56+ (percent)

Day 0148.718.063.62.712.2
269.213.881.23.67.9
338.817.657.64.019.5

FBS166.521.591.13.15.4
277.110.788.14.27.5
388.05.996.62.00.9

ABS161.423.486.93.59.1
272.112.983.64.611.0
389.75.696.51.21.0

HPL180.711.295.81.62.5
292.34.097.81.21.0
385.25.898.31.20.3

Phenotype of cells before expansion (day 0) and after 14 days of expansion using different supplements. Data represent percentage of positive cells. FBS: fetal bovine serum; ABS: AB negative serum; HPL: human platelet lysate.

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