Human Platelet Lysate Media Supplement Supports Lentiviral Transduction and Expansion of Human T Lymphocytes While Maintaining Memory Phenotype

Table 1

Results of immunostaining/flow cytometry following expansion.

	Donor	CD3^+CD4^+ (percent)	CD3^+CD8^+ (percent)	CD3^+CD56^-(percent)	CD3^+CD56^+ (percent)	CD3^-CD56^+ (percent)
Day 0	1	48.7	18.0	63.6	2.7	12.2
	2	69.2	13.8	81.2	3.6	7.9
	3	38.8	17.6	57.6	4.0	19.5
	FBS	1	66.5	21.5	91.1	3.1	5.4
2		77.1	10.7	88.1	4.2	7.5
3		88.0	5.9	96.6	2.0	0.9
ABS		1	61.4	23.4	86.9	3.5	9.1
	2	72.1	12.9	83.6	4.6	11.0
	3	89.7	5.6	96.5	1.2	1.0
	HPL	1	80.7	11.2	95.8	1.6	2.5
2		92.3	4.0	97.8	1.2	1.0
3		85.2	5.8	98.3	1.2	0.3

Phenotype of cells before expansion (day 0) and after 14 days of expansion using different supplements. Data represent percentage of positive cells. FBS: fetal bovine serum; ABS: AB negative serum; HPL: human platelet lysate.