Enhancement of Macrophage Function by the Antimicrobial Peptide Sublancin Protects Mice from Methicillin-Resistant Staphylococcus aureus
Effects of sublancin on the function of peritoneal cells and splenocytes in vivo. Sublancin enhanced the phagocytic activity of peritoneal macrophages ex vivo (a and b). Four- to six-week-old female BALB/c mice were separated into three groups: control, sublancin low-dose group (0.3 mg/kg or 0.6 mg/kg), and sublancin high-dose group (1.0 mg/kg or 1.2 mg/kg). The mice were orally administered with sublancin for 28 days (0.3 mg/kg and 1.0 mg/kg) or 14 days (0.6 mg/kg and 1.2 mg/kg). Mice in the control group were given distilled water by gavage. Peritoneal macrophages were harvested and incubated with fluorescent microspheres for the determination of phagocytic activity. (c) Comparison of TNF-α production in peritoneal macrophages stimulated with LPS (1 μg/ml). (d) Sublancin (1.2 mg/kg) promoted a weak level of NK cell activity. Splenocytes were isolated from mice and cocultured with Yac-1 cells by a ratio (splenocytes : Yac-1) of 75 : 1 for 4 h. Cytotoxicity was measured by the lactate dehydrogenase assay. (e) Comparison of IFN-γ level in activated splenic T cells (2 μg/ml anti-CD3 and 1 μg/ml anti-CD28 antibodies). Data shown are and derived from 4 to 5 mice in each group. Significant difference with the control group were designated as and .
Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.