The RNA-Binding Protein KSRP Modulates Cytokine Expression of CD4⁺ T Cells

Figure 3

Time kinetics of T cell-mediated cytokine production. In supernatants from 6 h, 24 h, 48 h, and 72 h polyclonally stimulated CD4⁺ T cells isolated by magnetic bead separation from KSRP^+/+ (WT) and KSRP^-/- (KO) mice, IL-5, ΙL-10, IL-13, and IL-9 contents were measured by CBA (a). Shown data are the ( animals per genotype) and are pooled from two technical replications ( and ; two-way Anova/multiple comparison test). (b) In CD4⁺ T cells from KSRP^+/+ (WT) and KSRP^-/- (KO) mice used for CBA analyses, mRNA expression of IL-5, IL-10, IL-13, IL-9, and IL-4 was measured by qRT-PCR 24 h and 48 h after polyclonal stimulation of the cells. The mRNA expression of the different cytokines was normalized to Pol2a or 18S rRNA mRNA expression. The mRNA expression of KSRP^+/+ mice polyclonally stimulated for 24 h was set to 100%. Shown data are the ( animals per genotype) and performed in duplicate (, , and ; two-way Anova/multiple comparison test).

(a)

(b)