Journal of Immunology Research / 2019 / Article / Fig 4

Research Article

The RNA-Binding Protein KSRP Modulates Cytokine Expression of CD4+ T Cells

Figure 4

Effect of KSRP knockdown on T cell-mediated cytokine production in CAIA-treated mice. KSRP+/+ (WT) and KSRP-/- (KO) mice were treated on day 0 with a cocktail of 5 collagen-II-specific mAbs (AB) or with PBS (Ctrl) as control. On day 3, the animals were treated with LPS (50 μg/animal) or PBS as control. At day 5 and day 9, mice () of each treatment group were killed for subsequent analyses. Nylon wool-enriched T cells isolated from the spleens of KSRP+/+ (WT) and KSRP-/- (KO) mice treated with (AB) or without (Ctrl) CII-specific mAbs were polyclonally stimulated with anti-CD3- (1 μg/ml) and anti-CD28- (2 μg/ml) specific antibodies for 96 h. IL-5, IL-10, IL-13, and IL-9 expression was measured in the supernatant of the cells using CBA (a). Data shown are the ( animals per genotype at days 5 and 9) and are pooled from four independent experiments ( and ; unpaired -test). From a subset of these cells, RNA was isolated and mRNA expression of IL-5, IL-10, IL-13, and IL-9 was determined (b). The mRNA expression of the different cytokines was normalized to Pol2a mRNA expression. The mRNA expression of KSRP+/+ mice was set to 100%. Data shown are the ( animals per genotype at days 5 and 9) and are pooled from four independent experiments ( and ; ns=not significant, unpaired -test).
(a)
(b)

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