C/EBP Homologous Protein (CHOP) Activates Macrophages and Promotes Liver Fibrosis in Schistosoma japonicum-Infected Mice

Figure 2

Expression of CHOP in transcription and protein levels increased after S. japonicum cercariae infection in murine liver homogenates. Mice were treated with S. japonicum cercariae or sterile 9% physiological saline; after being administrated for 6 and 10 weeks, 6-8 mice were euthanized. Real-time PCR, Western blot, and immunohistochemical assays were performed to detect the expression of CHOP in the liver homogenate. (a) The protein expression of CHOP detected by WB. (b) The mRNA expression quantitative analysis of CHOP in the liver homogenate. (c) The protein expression quantitative analysis of CHOP in the liver homogenate. (d) The quantitative analysis of CHOP in liver tissues. (e) The protein expression of CHOP detected by immunohistochemistry (magnified ×400). Data were presented as of 6-8 mice. and vs. the control. ^# and ^## vs. the 6-week infection group.

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