Detection by Flow Cytometry of Anti-DNA Autoantibodies and Circulating DNA Immune Complexes in Lupus Erythematosus

Figure 3

A serum sample of SLE with IC-eDNA and no detectable free anti-DNA antibodies. SS is the side scatter; FL3 is fluorescence of DNA stained by EB and FL1 fluorescence due to antibody labeling by FITC-conjugated antiglobulin. In the upper histograms, SLE serum sample incubated with ctDNA and in the lower histograms before incubation. IC-eDNA and some free DNA were already present in the serum, but after incubation with the ctDNA, the IC-DNA level did not increase significantly.