Research Article
The Postembryonic Development of the Immunological Barrier in the Chicken Spleens
Table 1
Primers and conditions used in the qPCR.
| | Target genes | GenBank accession | Primer sequences (5-3) |
| | TLR2 | XM015301380 | F:ATCCTGCTGGAGCCCATTCAGAG | | R:TTGCTCTTCATCAGGAGGCCACTC | | TLR4 | NM001030693 | F:AGTCTGAAATTGCTGAGCTCAAAT | | R:GCGACGTTAAGCCATGGAAG | | IL-2 | XM015276098 | F:CAAGAGTCTTACGGGTCTAAATCAC | | R:GTTGGTCAGTTCATGGAGAAAATC | | IFN-γ | NM205149.1 | F:GACAAGTCAAAGCCGCACA | | R:TCAAGTCGTTCATCGGGAGC | | TNF-α | XM015294125 | F:GGACAGCCTATGCCAACAAG | | R:ACACGACAGCCAAGTCAACG | | β-Actin | NM205518 | F:GAGAAATTGTGCGTGACATCA | | R:CCTGAACCTCTCATTGCCA |
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