Journal of Immunology Research / 2019 / Article / Fig 4

Research Article

Calcitriol Inhibits the Proliferation of Triple-Negative Breast Cancer Cells through a Mechanism Involving the Proinflammatory Cytokines IL-1β and TNF-α

Figure 4

Calcitriol and its analogue increased IL-1β and TNF-α gene expression in SUM-229PE cells. Cells were cultured in the absence or presence of different concentrations of calcitriol (black bars) or EB1089 (white bars) for 24 hours. (a) IL-1β and (b) TNF-α gene expression was assessed by qPCR. The results represent the average of at least 3 experiments in . vs. control (0). For data normalization, gene expression in cells without treatment was considered as one.

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