Calcitriol Inhibits the Proliferation of Triple-Negative Breast Cancer Cells through a Mechanism Involving the Proinflammatory Cytokines IL-1β and TNF-α

Figure 5

Calcitriol inhibited cell proliferation by inducing endogenous production of IL-1β and TNF-α. SUM-229PE cells were treated with calcitriol (10 nM), IL-1β (0.5 ng/ml), TNF-α (5 ng/ml), anti-IL-1R1 (5 ng/ml), and anti-TNFR1 (2 ng/ml), individually or combined as indicated. Cell proliferation was evaluated after 6 days of treatment using the XTT method. Vehicle-treated cells were considered as 100% of cell proliferation. The results represent the average of 3 experiments, each in . vs. vehicle-treated cells (-). calcitriol or cytokine alone vs. combined treatments.

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