Implementation of Mass Cytometry for Immunoprofiling of Patients with Solid Tumors

Figure 3

CD45-based barcoding. A single-cell suspension was prepared from tonsillar carcinoma, stained for live and dead cells, and halved for barcoding. After barcoding with CD45-89Y and CD45-156Gd antibodies, the samples were combined and stained with the rest of the antibodies. (a) Debarcoding and (b) dot plots with a selection of representative antigens. The same gates were applied to measured cells.

(a)

(b)