Implementation of Mass Cytometry for Immunoprofiling of Patients with Solid Tumors

Table 1

Panel of antibodies for mass cytometry.

#	Antigen	Isotope	Clone	Staining	Company
1	CD1c (BDCA-1)	166Er	AD5-8E7	Surface	Miltenyi Biotec
2	CD3	154Sm	UCHT1	Surface	Fluidigm
3	CD4	145Nd	RPA-T4	Surface	Fluidigm
4	CD8a	146Nd	RPA-T8	Surface	Fluidigm
5	CD11b (Mac-1)	209Bi	ICRF44	Surface	Fluidigm
6	CD11c	152Sm	Bu15	Surface	BioLegend
7	CD14	151Eu	M5E2	Surface	Fluidigm
8	CD15	173Yb	MEM-158	Surface	Exbio
9	CD16	148Nd	3G8	Surface	Fluidigm
10	CD19	142Nd	HIB19	Surface	Fluidigm
11	CD25	169Tm	2A3	Surface	Fluidigm
12	CD39	160Gd	A1	Surface	Fluidigm
13	CD45	89Y or 156Gd	HI30	Surface	Fluidigm
14	CD45RA	155Gd	HI100	Surface	Fluidigm
15	CD45RO	164Dy	UCHL1	Surface	Fluidigm
16	CD56 (NCAM)	176Yb	N901	Surface	Fluidigm
17	CD62L (L-selectin)	153Eu	DREG-56	Surface	Fluidigm
18	CD68	171Yb	Y1/82A	Intracellular	Fluidigm
19	CD69	144Nd	FN50	Surface	Fluidigm
20	CD127 (IL-7Rα)	149Sm	A019D5	Surface	Fluidigm
21	CD141 (BDCA-3)	165Ho	AD5-14H12	Surface	Miltenyi Biotec
22	CD152 (CTLA-4)	161Dy	14D3	Intracellular	Fluidigm
23	CD197 (CCR7)	159Tb	G043H7	Surface	Fluidigm
24	CD275 (ICOS-L)	163Dy	2D3/B7-H2	Surface	BD Biosciences
25	CD278 (ICOS)	143Nd	DX29	Surface	Fluidigm
26	CD279 (PD-1)	175Lu	EH12.2H7	Surface	Fluidigm
27	CD303 (BDCA-2)	147Sm	201A	Surface	Fluidigm
28	CD366 (Tim-3)	150Nd	F38-2E2	Surface	BioLegend
29	Foxp3	162Dy	259D/C7	Intracellular	Fluidigm
30	HLA-DR	170Er	L243	Surface	Fluidigm
31	Ki-67	172Yb	B56	Intracellular	Fluidigm

In-house conjugation with isotope tags applying the Maxpar Antibody Labeling Kit (Fluidigm).