Table 1: Panel of antibodies for mass cytometry.

#AntigenIsotopeCloneStainingCompany

1CD1c (BDCA-1)166ErAD5-8E7SurfaceMiltenyi Biotec
2CD3154SmUCHT1SurfaceFluidigm
3CD4145NdRPA-T4SurfaceFluidigm
4CD8a146NdRPA-T8SurfaceFluidigm
5CD11b (Mac-1)209BiICRF44SurfaceFluidigm
6CD11c152SmBu15SurfaceBioLegend
7CD14151EuM5E2SurfaceFluidigm
8CD15173YbMEM-158SurfaceExbio
9CD16148Nd3G8SurfaceFluidigm
10CD19142NdHIB19SurfaceFluidigm
11CD25169Tm2A3SurfaceFluidigm
12CD39160GdA1SurfaceFluidigm
13CD4589Y or 156GdHI30SurfaceFluidigm
14CD45RA155GdHI100SurfaceFluidigm
15CD45RO164DyUCHL1SurfaceFluidigm
16CD56 (NCAM)176YbN901SurfaceFluidigm
17CD62L (L-selectin)153EuDREG-56SurfaceFluidigm
18CD68171YbY1/82AIntracellularFluidigm
19CD69144NdFN50SurfaceFluidigm
20CD127 (IL-7Rα)149SmA019D5SurfaceFluidigm
21CD141 (BDCA-3)165HoAD5-14H12SurfaceMiltenyi Biotec
22CD152 (CTLA-4)161Dy14D3IntracellularFluidigm
23CD197 (CCR7)159TbG043H7SurfaceFluidigm
24CD275 (ICOS-L)163Dy2D3/B7-H2SurfaceBD Biosciences
25CD278 (ICOS)143NdDX29SurfaceFluidigm
26CD279 (PD-1)175LuEH12.2H7SurfaceFluidigm
27CD303 (BDCA-2)147Sm201ASurfaceFluidigm
28CD366 (Tim-3)150NdF38-2E2SurfaceBioLegend
29Foxp3162Dy259D/C7IntracellularFluidigm
30HLA-DR170ErL243SurfaceFluidigm
31Ki-67172YbB56IntracellularFluidigm

In-house conjugation with isotope tags applying the Maxpar Antibody Labeling Kit (Fluidigm).