Lipid-Rich Extract from Mexican Avocado Seed (Persea americana var. drymifolia) Reduces Staphylococcus aureus Internalization and Regulates Innate Immune Response in Bovine Mammary Epithelial Cells
The lipid-rich avocado seed extract did not affect S. aureus growth and bMECs viability. (a) S. aureus was grown in LB at 37°C (18 h). The OD590 was adjusted at 0.2 ( CFU) and the treatments were added. The growth was monitored measuring the OD at the indicated time. (b) bMECs were grown in 96-well dishes (80% confluence) and the treatments were added. The viability was determined by Trypan blue exclusion assay at 24 and 48 h. The results correspond to three independent experiments performed by triplicate. Vehicle (DMSO 0.1%) (, Student’s -test).
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