Lipid-Rich Extract from Mexican Avocado Seed (Persea americana var. drymifolia) Reduces Staphylococcus aureus Internalization and Regulates Innate Immune Response in Bovine Mammary Epithelial Cells
The lipid-rich avocado seed extract inhibits S. aureus internalization into bMECs and the adhesion. (a) The effect of LEAS on the internalization of S. aureus into bMECs is presented as the percentage of CFU recovered after the lysis of bMECs. The values were determined considering the vehicle (DMSO 0.1%) as 100% of internalization. The results are the average of three independent experiments performed in triplicate. Vehicle (DMSO 0.1%). indicates significant changes (, Student’s -test). (b) To determine the adhesion of S. aureus on bMECs, cells were cultured and treated as described in Materials and Methods but the gentamicin treatment was omitted. Data are presented as the percentage of adhesion in relation to control (bMECs treated with vehicle). indicates significant changes (, Student’s -test).
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