Automated and Reproducible Detection of Vascular Endothelial Growth Factor (VEGF) in Renal Tissue Sections

Figure 1

Identification of the objects: the pipeline of CellProfiler. (a) Original image. (b) Image (a) converted to grayscale. (c) Image (b) with inverted intensities. (d) Identified nuclei. (e) Identified nuclei. Different colors indicate different objects. (f) Identified cells. Different colors indicate different objects. (g) Identified cytoplasm (i.e., cellular area minus nuclear area). Different colors indicate different objects.

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