Research Article
Automated and Reproducible Detection of Vascular Endothelial Growth Factor (VEGF) in Renal Tissue Sections
Table 1
Pipeline programmed for image analysis with CP.
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(1) Load user-defined images. (2) Convert the original images to grayscale images. (3) Invert intensities to have bright nuclei. (4) Identify the primary object of interest (in this case, the nucleus). (5) Identify the secondary object (in this case, the entire cell). (6) Create the tertiary object (cytoplasm) by subtracting the primary object from the secondary object, i.e., subtracting the nucleus from the cell. (7) Analyze morphologic parameters in the object called cytoplasm. (8) Calculate intensity parameters in the object called cytoplasm. |