Research Article

STAT Family Protein Expression and Phosphorylation State during moDC Development Is Altered by Platinum-Based Chemotherapeutics

Figure 4

Expression and phosphorylation of STAT proteins and phenotype of moDCs during maturation in the presence or absence of platinum drugs. (a) Expression and phosphorylation of STAT1, STAT3, STAT5, and STAT6 on day 7 after maturation in the presence or absence of oxaliplatin (4 μg/ml or 7 μg/ml) or cisplatin (2.5 μg/ml or 5 μg/ml), with tubulin used as loading control. One representative band of 3 donors is shown. (b) Quantification of STAT expression and phosphorylation, normalized to tubulin, shown as relative expression to untreated control cells (). Data are depicted as . (c) Expression of HLA-ABC and HLA-DR/DQ/DQ and costimulatory molecules CD80 and CD86 by moDCs treated with oxaliplatin (4 μg/ml or 7 μg/ml) or cisplatin (2.5 μg/ml or 5 μg/ml) during maturation as compared to untreated moDCs. The MFIs of live cells are shown as mean fold of untreated control cells + SEM ( (TLR maturation) or (cytokine maturation)). MoDCs matured with TLR ligands and moDCs matured with cytokines are obtained from different donors. ; ; .
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