Research Article

Chlorogenic Acid Inhibits BAFF Expression in Collagen-Induced Arthritis and Human Synoviocyte MH7A Cells by Modulating the Activation of the NF-κB Signaling Pathway

Figure 4

CGA suppresses TNF-α-induced BAFF expression in MH7A cells. Cells were stimulated with 20 ng/ml TNF-α for 4 h and then treated with CGA (50 and 100 μM) for 20 h; the BAFF expression was analyzed by real-time quantitative PCR (a) and Western blot (b). DEX was used as a positive control, and α-tubulin was used as a loading control. The densitometry analysis for Western blot was provided. vs. DMSO, vs. TNF-α treated, and vs. CGA 50 μM+TNF-α treated.
(a)
(b)