Chlorogenic Acid Inhibits BAFF Expression in Collagen-Induced Arthritis and Human Synoviocyte MH7A Cells by Modulating the Activation of the NF-κB Signaling Pathway

Figure 5

CGA alleviates the binding activity of NF-κB to the BAFF promoter by suppressing the NF-κB pathway. (a) MH7A cells were transiently transfected with the luciferase reporter plasmid pBAFF/929, pBAFF/750, or pBAFF/mutant, followed by stimulation with 20 ng/ml TNF-α for 4 h, and then treatment with CGA (50 and 100 μM) for 20 h. The luciferase assay was performed. Data were from three experiments in triplicate. (b) Cells were treated as above; the nuclear extracts were prepared, and EMSA was performed. (c, d) MH7A cells were treated as above, and then, IκBα, phospho-IκBα (p-IκBα), and p65 were examined by Western blot. α-Tubulin was used as a loading control. The densitometry analysis for Western blot was provided. vs. DMSO, vs. TNF-α treated, and vs. CGA 50 μM+TNF-α treated.

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