Human Hepatocytes and Differentiated Adult-Derived Human Liver Stem/Progenitor Cells Display In Vitro Immunosuppressive Properties Mediated, at Least in Part, through the Nonclassical HLA Class I Molecule HLA-G

Figure 1

The in vitro immune inhibitory properties of human liver cells. Hepatocytes, undifferentiated ADHLSCs, or differentiated ADHLSCs were added as third-party cells to MLR cultures for 7 days. Liver cells were added to the MLR at the indicated MLR : cells ratios of 1 : 0.5, 1 : 0.25, and 1 : 0125. The proliferation of allogeneic responder PBMCs was evaluated by the [3H]-thymidine incorporation method. The proliferation obtained in MLR was arbitrarily assigned a value of 100% and used as a reference to calculate the percentage of proliferation obtained with the other conditions. The bars represent the of at least 3 independent experiments. The data were analyzed using the one-way ANOVA test followed by the Dunnett post hoc test for multiple comparisons of the MLR group to the other treatment groups. and .