Human Hepatocytes and Differentiated Adult-Derived Human Liver Stem/Progenitor Cells Display In Vitro Immunosuppressive Properties Mediated, at Least in Part, through the Nonclassical HLA Class I Molecule HLA-G

Figure 2

Analysis of HLA-G mRNA expression by human liver cells. Total RNA was extracted from hepatocytes as well as undifferentiated and differentiated ADHLSCs, and cDNA was synthesized and used in a real-time RT-PCR assay for primary HLA-G transcripts. The choriocarcinoma cell line Jeg-3 was used as a positive control of HLA-G mRNA expression. Results are expressed as the of three independent experiments and are depicted as relative quantities of the HLA-G transcript compared to that found in Jeg-3 (arbitrarily assigned a value of 1).