DKK2 Impairs Tumor Immunity Infiltration and Correlates with Poor Prognosis in Pancreatic Ductal Adenocarcinoma

Figure 4

DKK2 impacted the infiltration capability of immune cells. (a) Gene set enrichment analysis of DKK2 showed the potential relationship between DKK2 and PDAC immunity invasion. (b) Representative immunohistochemical samples of DKK2, CD8A, FOXP3, GATA3, and T-bet in PDAC tissue microarray. (c) Patients were divided into two groups according to the expression of DKK2, and the column bar graphs showed the correlation between CD8A, FOXP3, GATA3, T-bet, and DKK2. (d) PBMCs treated with siRNA were performed with migration assay and then were counted by flow cytometry, and the percent was displayed by the bar graph (e). (f) Representative flow cytometry profiles of T cell subtype were shown. CD4⁺ and CD8⁺ cells, which were gated on single cells and lymphocytes, were gated as depicted. Significant differences are indicated as , , and .

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