Journal of Immunology Research / 2019 / Article / Fig 4

Research Article

High Thymic Output of Effector CD4+ Cells May Lead to a Treg : T Effector Imbalance in the Periphery in NOD Mice

Figure 4

NOD CD4+Foxp3+ and CD4+Foxp3- cells survive similarly to B6 control cells in situ. Seven-week-old NOD or B6 mice were injected with BrdU i.p. every 12 hours for 3 days (days –3, -2, and -1). On days 0, 5, 10, and 20 postinjection, LN (a,c) and spleen (b, d) cells were collected and labeled with CD4, CD25, Foxp3, and BrdU antibodies. CD4+Foxp3+ (a, b) or CD4+Foxp3- (c, d) cells in the LN and spleen were gated and the percentages of BrdU+ cells were determined ().
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