Journal of Immunology Research / 2019 / Article / Fig 6

Research Article

High Thymic Output of Effector CD4+ Cells May Lead to a Treg : T Effector Imbalance in the Periphery in NOD Mice

Figure 6

Differences in NOD vs B6 CD4+ Treg and non-Treg homeostatic proliferation do not account for the decrease in Treg percentages in the periphery of NOD mice. Four- or 11-week-old NOD or B6 mice were injected i.p. with BrdU every 12 hours for 3 days (days –3, -2, and –1). On day 0 (i.e., 12 hours after final BrdU injection), LN (a, c) and spleen (b, d) cells were collected and labeled with antibodies for CD4, Foxp3, and BrdU. CD4+Foxp3+ (a, b) or CD4+Foxp3- (c, d) cells were gated and analyzed for BrdU incorporation ().
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