Research Article

New Insights on the Adjuvant Properties of the Leishmania infantum Eukaryotic Initiation Factor

Figure 4

Effect of recombinant LieIF protein on the expression of co-stimulatory molecules by J774A.1 macrophages. Macrophages were stimulated with recombinant LieIF (10 μg/mL) for 24 h, and the expression of CD80 and CD86 molecules was measured using FACS with the use of specific monoclonal fluorochrome-labeled antibodies. Macrophages stimulated with LPS (1 μg/mL) were used as the positive control while unstimulated cells were used as the negative control. The results are expressed as (a) median fluorescent intensity (MFI) and (b) percentage (%) of macrophages expressing CD80 and CD86 molecules. Data are presented as mean of three independent experiments. (c) Histogram overlays are representative of one experiment. indicates statistically significant differences as compared to the negative control.