Research Article
Immunogenic Evaluation of Ribosomal P-Protein Antigen P0, P1, and P2 and Pentameric Protein Complex P0-(P1-P2)2 of Plasmodium falciparum in a Mouse Model
Figure 4
Regulatory immune response after threefold immunization with the recombinant ribosomal P. falciparum proteins. Two weeks after each vaccination (denoted here as I, II, and III), the number of blood (a, b) and spleen (c, d) CD4+CD25+ and CD4+FoxP3+ cells was determined by flow cytometry. (e) Representative dot plots of CD4+CD25+ cells in peripheral blood of mice immunized twice with the P0, P1, P2, P0-(P1-P2)2, and MSP-119 proteins, as well as in the adjuvant control and untreated animals. Numbers in the dot plot quadrants indicate the CD4+CD25+ cell percentage of total gated lymphocytes. Each data is presented as the of six mice. The differences were analyzed statistically with the Kruskal-Wallis test followed by the Dunn multiple comparison test. Statistically significant in comparison to the adjuvant group, , . +Significantly different from the second immunization with the same protein, +, ++.
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