Dendritic Cells Loaded with Heat Shock-Conditioned Ovarian Epithelial Carcinoma Cell Lysates Elicit T Cell-Dependent Antitumor Immune Responses In Vitro

Figure 3

Activation of allogeneic CD4⁺ T cells by monocyte-derived DCs maturated with heat shock-conditioned OEC lysates. Peripheral blood lymphocytes (PBL) were kept alone (LT alone) or cocultured for 5 days with allogeneic IL-4/GM-CSF-activated monocytes unstimulated (AM) or stimulated for 24 hours with 1 μg/mL LPS (LPS-DC) or 100 μg/mL of heat shock-conditioned lysates TRIMEL (TRIMEL-DC) and MOVL1 or MOVL2 (MOVL1-DC and MOVL2-DC, respectively). The expression of CD25, IFN-γ, IL-4, and IL-17 was evaluated in the CD4⁺ T cells by flow cytometry. The results were obtained from multiple independent experiments. Statistics compare treatments to LT alone; , , and ^#. Also, a comparison between MLOV1, LPS, and AM was made using the same values.