Journal of Immunology Research / 2020 / Article / Fig 2

Research Article

Adenosine-Induced NLRP11 in B Lymphoblasts Suppresses Human CD4+ T Helper Cell Responses

Figure 2

Adenosine-induced NLRP11 protein synthesis is de novo. (a) Daudi cells were left untreated or were treated with 10 μg/ml CHX and then mock stimulated or stimulated with adenosine for 4 hours. (b) Cells were treated with 50 μM uridine or cytidine for 4 hours. (c) NLRP11 mRNA and protein expression. (d) IL-1β and caspase-1 protein expressions after treatment with 50 μM caffeine for 4 hours. Cell lysates were separated by SDS-PAGE and immunoblotted (IB). Results are representative of three independent experiments. Student’s -test shows the significant difference between stimulated and nonstimulated cells. indicates , , and .

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