CD40 Signaling Promotes CXCR5 Expression in B Cells via Noncanonical NF-κB Pathway Activation

Figure 2

CD40-mediated CXCR5 expression was B cell intrinsic. (a) Representative flow cytometry data plots showed the coexpression of CXCR5 and CD40 on freshly isolated splenocytes or CD19⁺ cells (representing three independent experiments, ); (b) The purity of B cells sorted by the magnetic cell sorting was measured; (c, d) The purified B cells or total splenocytes were stimulated with anti-mouse CD40 antibody for 72 hours. In the transwell system, CD19^- splenocytes from normal mice were cultured in the upper chambers, while CD19⁺ cells were cultured in the lower chambers. (c) Representative flow cytometry data plots represent the frequencies of CXCR5⁺ cells within the CD19⁺ cell population (representing three independent experiments, ); (d) Data are representative of three independent experiments, ; Living cells were gated according to FSC and SSC parameters. All flow cytometry results were analysed and plotted using FMO. (ANOVA test).

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