Differential Immunogenicity and Protective Efficacy Elicited by MTO- and DMT-Adjuvanted CMFO Subunit Vaccines against Mycobacterium tuberculosis Infection

Figure 3

CMFO-specific T cells in the spleen and lung of different vaccinated mice before and after exposure (). 10 weeks after vaccination and 4 weeks postchallenge, splenocytes and lung cells were prepared from each mice and counted, respectively. The absolute numbers of CMFO-specific IFN-γ⁺ or IL-2⁺ CD4⁺ and CD8⁺ T cells, or T_CM cells secreting IL-2 and T_EM cells secreting IFN-γ per spleen and per lung were identified by intracellular cytokine staining and a multicolor flow cytometer. The results were shown as the . and .