Berberine Improves Inflammatory Responses of Diabetes Mellitus in Zucker Diabetic Fatty Rats and Insulin-Resistant HepG2 Cells through the PPM1B Pathway

Figure 8

Effect of BBR on the protein expression level of targets in the PPM1B pathway in HepG2-IR cells. (a) Representative Western blotting results of targets. (b–i) Semiquantitative analysis of PPM1B, LRP1, pIKKβ Ser181, GLUT4, PPARγ, IKKβ, NF-κB p65, and JNK in liver tissues. The experiments were repeated three times and presented as . ^▲ and ^▲▲, Con vs. model; and , model vs. BBR-treated groups. The HepG2-IR cells under the indicated treatment were costained by (j) PPM1B (green) and PPARγ (red) immunofluorescence antibody, as well as (k) LRP1 (green), (l) GLUT4 (green), (m) IKKβ (green), and (n) NF-κB p65 (green). DAPI (blue) was used to visualize nuclei. Representative microscopic pictures are shown. Magnification, ×100. Scale bar, 100 μm. The experiments were replicated at least three times.

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