Oral Immunotherapy Using Probiotic Ice Cream Containing Recombinant Food-Grade Lactococcus lactis Which Inhibited Allergic Responses in a BALB/c Mouse Model

Figure 4

PCR products of 3 colonies with modified vectors growth on M17 agar. Lane No.: ladder 100 bp plus 3 kb (DM2300), lanes 1-3 contributed to the vectors which extracted, lane 4: negative control of PCR with specific primers (pNZ7025-F and pNZ7025-R), lane 5: positive control for PCR with specific vector primers. Lanes 6-8 contributed to PCR products of extracted vector with specific pNZ7025 primers, lane 9 is a positive control for PCR with SDM-F3 and SDM-R2, lane 10 is a negative control for PCR with SDM-F3 and SDM-R2, and lanes 11-13 are PCR products of 3 modified vector with SDM-F3 and SDM-R2.