Corrigendum

Corrigendum to “Multicentre Harmonisation of a Six-Colour Flow Cytometry Panel for Naïve/Memory T Cell Immunomonitoring”

Table 1

Participants, instruments, and software. Five centres, with a total of 13 operators (including the reference operator, ROP), using seven different flow cytometers dedicated to research use (except GMP-maintained BD FACSCanto™ I at ISS, with a fluidic system upgrade, comparable to a BD FACSCanto™ II), participated to the harmonisation panel. Three flow cytometer models with compatible optical configuration (BC Gallios™, BD FACSCanto™ II, and BD LSR Fortessa™) were used. The data generated were analysed by operators at peripheral sites (local analysis) using their own analysis software (Kaluza, FlowJo, or FACSDiva). Central analysis at ISS was performed by the ROP with Kaluza software on local raw data (acquired fcs files). One of the two BD LSR Fortessa instruments, used by one operator from SUR center, belongs to the core facilities of the CLNS: Center for Life NanoScience@Sapienza, Istituto Italiano di Tecnologia, Rome, Italy.

5 centres13 operators7 instruments3 instrument models2 acquisition software3 analysis software

ISS
SUR
INMI
IRE
OPBG
12+1 ROP1 FACSCanto I+
(GMP)
3 BD FACSCanto II
2 BD LSRFortessa
1 BC Gallios
BD FACSCanto II BD LSRFortessa
BC Gallios
BC Kaluza
BD FACSDiva
BC Kaluza
BD Diva
TreeStar
FlowJo