Research Article
Evaluation of the Ability of Miltefosine Associated with Topical GM-CSF in Modulating the Immune Response of Patients with Cutaneous Leishmaniasis
Figure 4
Influence of miltefosine and GM-CSF treatment in the phagocytosis and killing of L. braziliensis by monocytes from CL patients. Monocytes from CL patients treated with miltefosine + GM-CSF (), miltefosine + placebo (), and Sbv () were infected with L. braziliensis promastigotes at a 5 : 1 ratio for 2, 48, and 72 hours. The percentage of infected cells after 2 hours (a), 48 hours (b), and 72 hours (c) as well as the number of intracellular parasites after 2 (d), 48 (e), and 72 (f) hours were determined by optical microscopy, after panoptic staining on day 0 and day 15 of therapy. The data is represented with median and interquartile range. Statistical analyses were performed using Mann-Whitney’s test for unpaired groups and Wilcoxon’s rank test for paired measurements; , .
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