Attenuated Subcomponent Vaccine Design Targeting the SARS-CoV-2 Nucleocapsid Phosphoprotein RNA Binding Domain: In Silico Analysis

Table 4

MHC-II epitopes of SARS-CoV-2 NP.

S/N	Start	End	Peptide	Length	Core sequence	Alleles	Antigenicity	Conservancy	Toxicity	Allergenicity
1	107	121	YYLGTGPEA	15	RWYFYYLGTGPEAGL	—	0.7969	100	Nontoxic	Allergen
2	325	339	LTYTGAIKL	15	TPSGTWLTYTGAIKL	HLA-DRB107:01, HLA-DRB501:01, HLA-DRB115:01, HLA-DRB302:02	0.6524	100	Nontoxic	Nonallergen
3	106	120	FYYLGTGPE	15	PRWYFYYLGTGPEAG	—	1.1904	100	Nontoxic	Allergen
4	314	328	FGMSRIGME	15	FFGMSRIGMEVTPSG	HLA-DRB401:01, HLA-DRB302:02, HLA-DRB103:01, HLA-DRB115:01	0.9467	100	Nontoxic	Nonallergen
5	222	236	LNQLESKMS	15	LLLDRLNQLESKMSG	—	0.6213	100	Nontoxic	Allergen
6	387	401	VTLLPAADL	15	KKQQTVTLLPAADLD	HLA-DRB401:01, HLA-DRB501:01, HLA-DRB107:01, HLA-DRB115:01	0.6417	100	Nontoxic	Nonallergen

Selected HTL epitopes. Selected epitopes are tagged as H2, H4, and H6.