Analysis of the Heterogeneity of CD4⁺CD25⁺ T Cell TCR β CDR3 Repertoires in Breast Tumor Tissues, Lung Metastatic Tissues, and Spleens from 4T1 Tumor-Bearing BALB/c Mice

Table 1

Analysis of composition and characteristics of frequency for the top five sequences and the usage of TRBV and TRBJ gene, CDR3 AA of CD4⁺CD25⁺ T cells TCR β CDR3 repertoires in breast tumor tissues, lung metastatic tissues, and spleens from 4T1 tumor-bearing BALB/c mouse 1.

	TRV gene	TRJ gene	CDR3 sequences (AA)	Frequency (%)	Total frequency (%)
M1-B (top5)	TCRBV31-01	TCRBJ01-05	CAWRGTFHNNQAPLF	1.181	3.983
	TCRBV13-01	TCRBJ02-07	CASSERLGGYEQYF	1.099
	TCRBV02-01	TCRBJ01-05	CASSQGQGANQAPLF	0.741
	TCRBV29-01	TCRBJ01-02	CASSLGTGNSDYTF	0.483
	TCRBV31-01	TCRBJ02-07	CAWSPGLGGRDEQYF	0.479
M1-L (top5)	TCRBV13-01	TCRBJ02-07	CASSERLGGYEQYF	0.531	1.980
	TCRBV02-01	TCRBJ01-05	CASSQGQGANQAPLF	0.427
	TCRBV31-01	TCRBJ01-05	CAWRGTFHNNQAPLF	0.375
	TCRBV13-02	TCRBJ02-01	CASGGDWGGNYAEQFF	0.323
	TCRBV13-02	TCRBJ01-04	CASGDISNERLFF	0.323
M1-S (top5)	TCRBV20-01	TCRBJ02-01	CGARPWGGAEQFF	0.309	0.769
	TCRBV31-01	TCRBJ01-05	CAWRGTFHNNQAPLF	0.159
	TCRBV19-01	TCRBJ01-04	CASSTRTSNERLFF	0.109
	TCRBV04-01	TCRBJ02-01	CASSFRDRGYAEQFF	0.100
	TCRBV17-01	TCRBJ01-03	CASSSRTGSGNTLYF	0.092