ChIP-seq Profiling Identifies Histone Deacetylase 2 Targeting Genes Involved in Immune and Inflammatory Regulation Induced by Calcitonin Gene-Related Peptide in Microglial Cells
Cultured mouse microglial cells (BV2) expressed Iba1 and CGRP receptor components CRLR, RAMP1, or CRCP. (a) Expression of Iba1 (a marker of microglia, red) and its colocalization with CRLR, RAMP1, or CRCP staining (green) in cultured microglial cells. Scale bar 40 μm. (b) Western blot analysis of HDAC2 expression in microglia with treatment with CGRP at 0, 1, 2, 4, and 6 h, respectively. Relative amounts of proteins were calculated by normalizing to GAPDH. Data are presented as the ( independent cell culture preparations, Mann-Whitney tests or Kruskal-Wallis tests). .
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