Comprehensive Screening of Mouse T-Cell Epitopes in Human Herpesvirus 6B Glycoprotein H/L/Q1/Q2 Tetramer Complex

Figure 1

T-cell responses of mice immunised with the tetrameric protein complex plus CpG adjuvant. Undepleted whole splenocytes (a, d), CD8⁺ T-cell-depleted splenocytes (b, e), and CD4⁺ T-cell-depleted splenocytes (c, f) of BALB/c (a–c) and C57BL/6 (d–f) mice that had been immunised with HHV-6B gH/gL/gQ1/gQ2 tetrameric protein complex plus CpG adjuvant were tested for reactivity to a library of 162 peptides after 20 h of stimulation by IFN-g ELISA. The quantity of IFN-γ (pg/mL) of each peptide was transformed to a score (, where is the quantity of IFN-γ for each peptide). Individual peptide samples with a score exceeding 0.01 were considered positive. Black bars indicate that the response was detected as positive in all three independent experiments, and gray bars indicate that there was positivity in two out of three independent experiments. T-cell responses detected as positive in only one experiment are not shown. Data are shown as the mean of three independent experiments. The gray dot lines indicate the boundaries of gH, gL, gQ1, and gQ2.