Comprehensive Screening of Mouse T-Cell Epitopes in Human Herpesvirus 6B Glycoprotein H/L/Q1/Q2 Tetramer Complex

Table 2

CD8⁺ T-cell short epitope peptide prediction in C57BL/6 mice.

Peptide no. (glycoprotein)	Length (aa)	Amino acid sequence1	Estimated scores for restriction molecules^2		%rank
			BIMAS		NetMHC
			D^b	K^b	D^b	K^b
No. 133 (gQ1)	20	QRGTSIRNIDPAIVSALWHS
	9	IDPAIVSAL	0.017	3.3	26	18
	8	DPAIVSAL	—	1.1	65	70
	8	RGTSIRNI	—	0.475	48	30
	10	NIDPAIVSAL	0.05	0.24	27	46
	8	RNIDPAIV	—	0.174	48	19
	10	RNIDPAIVSA	0.119	0.158	55	50
	9	AIVSALWHS	0.022	0.132	35	40
	9	TSIRNIDPA	1047.388	0.12	0.01	11
No. 141 (gQ1)	20	MFEKKIYGYEHLGQALCEGG
	8	KIYGYEHL	—	132	9	0.03
	10	YGYEHLGQAL	3.024	8.64	1.2	1.8
	9	KKIYGYEHL	0.899	6	4.5	5
	10	EKKIYGYEHL	0.011	1.2	13	22
	8	YEHLGQAL	—	1	21	43
	9	YGYEHLGQA	0.108	0.72	7.5	3.5
	9	GYEHLGQAL	0.006	0.24	35	34
	8	EHLGQALC	—	0.11	95	80
No. 157 (gQ2)	20	NGPRYLQMETFISDLFRYEC
	8	NGPRYLQM	—	103.68	23	4.5
	10	LQMETFISDL	15.459	1.32	3.5	6
	8	METFISDL	—	1.1	37	28
	10	NGPRYLQMET	0.108	0.432	36	60
	10	YLQMETFISD	0.059	0.3	28	43
	8	LQMETFIS	—	0.264	39	37
	9	QMETFISDL	1.175	0.24	16	2.5
	10	RYLQMETFIS	0.003	0.22	4	36

¹ Underlined peptides were synthesised and used for experiments. Bold type peptides indicate determined short CD8⁺ T-cell epitope. ² No binding score.