B7-H4 Inhibits the Development of Primary Sjögren’s Syndrome by Regulating Treg Differentiation in NOD/Ltj Mice
B7-H4 on SGEC is involved in Treg differentiation. (a, b) Naïve CD4+ T cells were isolated from NOD/Ltj mice and cultured ( cells/well) with plus an equal number of SGECs in the presence of IL-2 (30-50 U/ml), TGF-β (2 ng/ml), plus IgG isotype, or anti-B7-H4 mAb. The cells were collected on day 3 of culture, and the percentage of CD4+FoxP3+/CD4+ T cells was determined by FACS analysis (a). Accumulated data of Tregs differentiation in vitro was shown (b). (c, d) Naïve CD4+ T cells from NOD/Ltj mice were activated in Treg-promoting conditions as detailed in (a, b) with control Ig or B7-H4 Ig, and the percentage of CD4+FoxP3+/CD4+ T cells was determined by FACS analysis (c). Accumulated data of Treg differentiation in vitro was shown (d). Two-tailed Student’s -test. All the data presented were from three independent experiments.
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