Research Article

Gastrospheres as a Model of Gastric Cancer Stem Cells Skew Th17/Treg Balance toward Antitumor Th17 Cells

Figure 1

Gastrospheres’ conditioned medium limits PBMC proliferation. (a) Monolayer culture of MKN-45 gastric cancer cell line and its derived-loose grape-like shape gastrospheres. (b) Colony size of PBMCs in exposure to MKN-45 parental cells or gastrosphere-derived conditioned media in MLR. (c, d) PBMCs’ proliferation in exposure to parental or gastrospheres’ conditioned media in MLR (). (e) PBMCs’ proliferation in exposure to parental or gastrospheres’ conditioned media in MLR at ratio 1 : 5. (f, g) PBMCs’ proliferation in exposure to parental or gastrospheres’ conditioned media in the presence of anti-CD3/CD28 microbeads at ratio 1 5. CSC: gastrospheres. Data are of three independent experiments. .
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