HMGB1 Recruits TET2/AID/TDG to Induce DNA Demethylation in STAT3 Promoter in CD4⁺ T Cells from aGVHD Patients

Figure 5

The overexpression of STAT3 induced by HMGB1 could be partially disrupted in CD4⁺ T cells transfected with the TET2/AID/TDG interference plasmid. (a) Representative western blot results for HMGB1, TDG, AID, TET2, STAT3, and GAPDH levels. (b) Quantitative analysis of the relative protein levels of HMGB1, TDG, AID, TET2, and STAT3. (c) DNA methylation levels of STAT3 promoter in CD4⁺ T cells transfected with different plasmids (control plasmids; HMGB1 overexpression plasmids; HMGB overexpression plasmids and TET2/AID/TDG interference plasmids); (, ). Data are presented as the of three independent experiments. , , .

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